Exhibit 23.1
CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM
We consent to the reference to our firm under the caption Experts in the Registration Statement
(Form S-3) and related Prospectus of Ulta Salon, Cosmetics & Fragrance, Inc. for the registration
of 11,222,529 shares of its common stock and to the incorporation by reference therein of our
reports dated March 31, 2010, with respect to the consolidated financial statements of Ulta Salon,
Cosmetics & Fragrance, Inc. and the effectiveness of internal control over financial reporting of
Ulta Salon, Cosmetics & Fragrance, Inc., included in its Annual Report (Form 10-K) for the year
ended January 30, 2010, filed with the Securities and Exchange Commission.
/s/ Ernst & Young LLP
Chicago, Illinois
June 3, 2010